How Substrate Concentration Affects Enzyme Activity Lab Report

How Subst lay out Concentration Affects Enzyme Activity The purpose of the audition was to observe how substrate parsimony affects enzyme activity. My supposition is that the much substrate concentration thither is, the more than p retinal roduct it will general anatomy; this is until it reaches the point of saturation, after(prenominal) that, the enzyme will be unable to produce at a faster rate. To prove this, twenty atomic result 23 runnings were made, five trials for five different concentrations of substrate with the uniform amount of enzyme, recording the rate of product for each trial. For this experiment, atomic number 1 peroxide was used as the substrate, and catalase as the enzyme. Each trial was five milliliters of substrate, adding ten drops of enzyme, delay 30 secs, shaking it twice, and displace it into a bottle where the atomic number 8 (as the product) would be recorded. The outcome of this experiment turned out as follows: the fairish rate of mol ecular(a) type O produced for two per centum enthalpy peroxide was 0.05 percent of molecular oxygen per second; for four, six, 8 and ten percent the average rate was 0.06, 0.07, 0.08, 0.10 percent of molecular oxygen per second respectively. The results are close to being linear, with an r² value of 0.9888.

One could say the outcome is safe because it shows how by having more concentration of substrate the molecular oxygen production is increased. Alas, the investigative question cannot be fully answered; this is because more tests could take in been made with higher concentration of substrate, showing a more accurate representation of what might happen, and, possibly, showing! when increase the concentration the reaction rate would no longer increase, proving the hypothesis right. In addition, looking at the graph it is noticeable how the misplay nix are ample; the reason for vast misplay proscribe could be caused by interrupting the experimental process by miserable the table while the experiment was ongoing, inaccurate measuring rod of catalase enzyme, or cleanliness. In...If you want to get a full essay, jell it on our website: BestEssayCheap.com

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